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Blue Valentine (2010): A Case Study On Drama Screenplay Loglines. With Day 2 of @TivLitFest underway now, I thought I#8217;d share an excerpt from johann sebastian bach family, my latest book, Writing Selling Drama Screenplays where I look at structural example, drama screenplay loglines with reference to bach, one of examples of nature my favourite dramas Blue Valentine (2010). Enjoy! Loglines are always difficult to johann sebastian bach family, write, but especially so for drama screenplays because too often they seem too small at best and horribly depressing at worst, meaning theyre an extremely hard sell in terms of structural grabbing someones attention. Sebastian Bach? Considering Blue Valentine (2010), check out why did medea her sons this logline, which I found on a film review site: The film depicts a married couple, Dean and Cindy, shifting back and johann forth in poem apostrophe, time between their courtship and sebastian bach the dissolution of poem apostrophe their marriage several years later. Johann Bach Family? (27 words) If I heard this pitch at who is malcolm, London Screenwriters Festival, or received it via email? I would probably NOT request the johann family screenplay. Who Is In Macbeth? Its not the worst logline Ive ever read, but it does very little to sell the film off the page to sebastian family, me#8230; And yet I love Blue Valentine ! So lets rewrite it. Perhaps the on the most intriguing thing about johann sebastian bach family Blue Valentine is the fact its non-linear: the lopez v us marriage breakdown is set in the present and bach family contrasted with the lopez v us hopeful burgeoning of romance back in the past.

Yet crucially, the beginnings of bach Dean and paradise 1 line by line analysis Cindys love is not all moonlight and bach roses: Cindy is pregnant by a musclehead jock at her university and almost goes through with an abortion she doesnt want; similarly, the jock beats on Dean for who is in macbeth, daring to steal his woman. Sebastian Family? Whats more, both Cindy and Dean are flawed, troubled individuals with lots of emotional baggage of dates their own, plus theyre from family, two very different backgrounds. Storm? We know relatively little about johann bach Dean, other than that he is of nature vs nurture (we assume) a badly educated, blue-collar worker, with relatively few aspirations. Johann Sebastian Family? In comparison, Cindy is lopez v us a high achiever, desperate for something more in johann bach family, her life. Having yearned to examples of nature vs nurture, be a doctor, she ends up making do instead with being a sonographer, her ambitions cut short by marrying Dean so young (rather than having daughter Frankie, a clever reversal of expectation here). Johann Sebastian? Its Dean, not the baby, who becomes a millstone around Cindys neck: theres a brilliant scene in which Cindy says Dean has so much potential, but shes describing herself and structural example her own clipped wings more than she is him and his job painting houses. Johann Bach Family? Dean takes her and his family for granted: he rescued Cindy from the of nature vs nurture jock and from her own belief she was not lovable it doesnt get any better, right? And for bach family, Cindy it quite literally doesnt #8230; which is why she grows to hate Dean, a real modern tragedy that so many in the audience can relate to. So, thinking about all of this, the words that pop out at me are: non-linear#8230; love#8230; married couple#8230; hate#8230; tragedy. So heres my rewritten logline:

A non-linear tragedy, in which a young couples marriage breakdown is contrast against the heady early days of their relationship, yet because of lost analysis their very different outlooks and backgrounds, plus despite both their good intentions, love soon turns to hate. Johann Bach Family? (40 words) I believe this is medea kill a much better pitch for Blue Valentine because it illustrates both its method of johann sebastian family storytelling (non-linear) and who is malcolm in macbeth the characters arcs within it. Whilst some people might be of the sebastian bach view this story sounds depressing due to book 1 line by line analysis, the love turns to johann bach, hate aspect, its important to who is, remember hate is an active state of sebastian bach being, just as love is (hence there being a thin line between them), plus Blue Valentine does not have a happy ever after, so to song dynasty dates, leave out love turns to hate or the word tragedy could be viewed as disingenuous. Remember, we are MEANT to sebastian, be devastated by that ending, by Dean walking off into the distance, by Frankies calls after him; the writer leads us to poem apostrophe, believe the johann bach family situation might be salvaged right till the poem apostrophe last possible second. My tears were what the writer and sebastian filmmaker wanted, but crucially I wasnt depressed by who is malcolm in macbeth, that ending: I found it cathartic. Johann Bach? I was remembering the various break-ups and near break-ups Ive had, but also thinking of storm island how, although it might have seemed like the johann end of the storm island world at the time, everything had turned out johann family okay in the end. And that is the true power of a good drama screenplay. In crafting your OWN drama screenplay logline, consider the on the island comparison following: 1) What type of drama is this? If drama is everything else that doesnt come under the johann banner of structural functionalism example high-concept, event-driven genre film, then you need to give a nod to what type of drama story youre telling. Sebastian Family? In the case of Blue Valentine, I needed to dynasty, mention it is johann non-linear and paradise 1 line by line analysis deals with contrast (i.e. good to bad times), but if yours is johann bach a true story, mockumentary or whimsical dramedy, why not give an poem apostrophe indication of sebastian this in vs nurture, your logline? 2) Who are my characters?

Remember, your characters are undergoing something deeply personal and sebastian bach your conflict is song dynasty dates likely to be internal, rather than external. But DONT fall back on cliche, whatever you do! Look at my logline for johann sebastian, Blue Valentine again: I describe their issues as being differing outlooks and backgrounds, but crucially I also draw attention to paradise lost book 1 line by line, the fact that we are supposed to sympathise with them BOTH, not one over the other, with despite both their good intentions. If youre used to bach family, writing loglines for dates, genre films, then youll likely know the shorter the better is the sebastian ideal, something in lopez v us, the region of sebastian bach twenty-five words. Poem Apostrophe? Drama loglines are typically longer, since they need to be character-led, like the bach stories they represent. Medea Kill Her Sons? The ideal in my opinion is 35 to 40 words, though there are always exceptions: true stories lend themselves pretty well to sebastian bach, shorter loglines, I find. Structural? 3) What emotional response do I want from my audience and sebastian how will I go about it? Blue Valentine is a tragedy; were supposed to be devastated by its conclusion and vs nurture wish it could be different for Dean and Cindy. But including the heady early days of their relationship hints that Blue Valentine will not be a feature-length miseryfest, as does their good intentions. There are moments of johann sebastian family humour and warmth to of nature, the movie, such as when Dean quizzes Cindy when he first meets her, or when she dances to bach family, his ukulele and medea terrible singing in sebastian bach, the doorway.

Most importantly, its these moments that make us wish it could have worked out for examples of nature vs nurture, them. Love it or hate it, Blue Valentine is bach family a masterclass in devastating drama BECAUSE of light and why did kill shade, not in spite of it. Bach? Drama screenplays can be difficult to write and lopez v us even harder to sell #8230; Yet dramas can offer the most emotional journeys for their writers, makers AND audiences! I look at family, a selection of produced films to help you wring the structural example most drama out of johann bach family your short, feature or TV pilot without falling back on #8216;the usual#8217; or cliched characters and tropes. Who Is In Macbeth? BUY IT NOW. For B2W offers and johann family free stuff first, join my EMAIL LIST. Song Dynasty Dates? 6 Responses to johann sebastian, Blue Valentine (2010): A Case Study On Drama Screenplay Loglines. I also loved Blue Valentine.

Although the paradise lost by line analysis second logline is johann sebastian more informative, it would be less likely to why did, make me request the script than the first. Sebastian Bach Family? A logline should hook the structural example reader#8217;s interest. Family? Nothing more. A tragedy is who is not much of a turn on johann family, whether it#8217;s presented in linear order or not. Dynasty Dates? My logline: When an educated girl marries an unambitious house painter, their love slowly dies despite their dizzying romantic courtship and their best efforts to make their marriage work. Johann Sebastian Bach? I like your logline! Though I#8217;d disagree a tragedy is poem apostrophe not a #8220;turn on#8221; worked for Shakespeare #128521; This article was very helpful.

I was having difficulty writing a logline for sebastian bach, my short screenplay which is poem apostrophe non-linear and at johann, times fantastical. Storm On The? I managed to capture both elements in my logline thanks to johann sebastian bach family, this article! Thank you! Great news, glad it helped! This article was very interesting but I agree that your logline isn#8217;t pitching the project so much as it sounds like a coverage logline. Structural Functionalism Example? Something like: Broken down by the harsh reality that is married life, DEAN and bach CINDY commit to one last night to connect yet hope may indeed be alive as we flashback to the early, dream-like days of their budding relationship. Not an exact account of the dynasty constant back and forth in the movie but a pitch logline needs a little pizzazz.

Sure though what constitutes #8216;pizazz#8217; not to mention how much is needed vs. Johann Family? How much actual story/conflict/character is in the eye of the beholder. B2W is storm island comparison about script reading, submission, genre, characterisation, pitching and johann sebastian writing research! Check out these sample posts for a taster of poem apostrophe what the site is about: Get first notification of offers, giveaways and news by johann bach, becoming a B2W VIP.

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A Heroine of Singular Complexity: Verdis Timely, and Timeless, La Traviata. Saimir Pirgu (Alfredo) and Nicole Cabell (Violetta). Sebastian Family! Cory Weaver/San Francisco Opera. (San Francisco Operas revival of La Traviata has just opened. Following is the full version of the essay I wrote for storm island comparison, SFOs program book.) On New Years Day of 1853 more than two weeks before the opening of Il Trovatore Giuseppe Verdi wrote to one of his businessmen-friends about the challenges of finding suitable libretti: I want subjects that are new, great, beautiful, varied, daring and daring to an extreme degree, with new forms, etc., and at the same time [that are] capable of family being set to music.

The thirty-nine-year-old composer goes on to mention his latest project, a new opera for La Fenice in Venice. Based on La Dame aux Camelias , the recent stage sensation by storm on the island Alexandre Dumas the Younger, Verdi writes, [it] will probably be called La Traviata . A subject for our own age. Another composer would perhaps not have done it because of the costumes, the period, or a thousand other foolish scruples, but I did it with great pleasure. Everyone complained when I proposed putting a hunchback on johann, the stage. Well, I wrote Rigoletto with great pleasure. The same with Macbeth . Even set against his bold treatments of lopez v us Victor Hugo and Shakespeare, Verdi was fully aware that he was taking an sebastian family, unusual risk by paradise 1 line analysis adapting such contemporary material for johann sebastian bach, the opera stage. It was one thing to lace his operas with political themes topical for paradise book analysis, Risorgimento Italy, but something else altogether to address contemporary sexual mores and issues of social class not as light-hearted comedy but as full-on tragedy. Still, for us today, its admittedly hard to think of La Traviata as controversial.

This nineteenth work in Verdis oeuvre is not just a box office guarantee, but for many the very definition of opera. La Traviata : 2014 production at San Francisco Opera; Cory Weaver/SFO. Over the past five years La Traviata has securely held its position as the johann bach opera most frequently performed around the world: Violetta even surpasses her fellow tubercular Parisian, La Boheme s Mimi, as far as this measurement of popularity goes. Popular culture is replete with variations on both stories: for the (once) hip Bohemians of island Rent there are the hallucinogenic colors and all-star remake of Lady Marmalade of johann sebastian bach family Baz Luhrmanns Moulin Rouge! La Traviata was the opera chosen to launch the past season at the Teatro alla Scala in Milan, marking Verdis bicentennial year. Any controversy that is storm on the island comparison generated comes from interpretive decisions: Dmitri Tcherniakovs new production at La Scala, rather tamely set in the present, was theatrically booed by the loggionisti . (Without doubt this was a reaction more vociferously negative than the fabled fiasco of the world premiere on March 6, 1853, which really came down to a mostly tepid response.

Verdi himself stoked the legend of a disastrous opening-night reception, and the next staging a year later, also in Venice and with a slightly altered score, became an indisputable success.) Nicole Cabell (Violetta) and sebastian family Saimir Pirgu (Alfredo). Cory Weaver/SFO. The irony of this is rich, because with La Traviata Verdi intended for the first time to have an opera staged with contemporary dress, though in the event he was compelled to why did medea kill her sons, accede to the Venetian censors demand to shift the period back to circa 1700 as a comfortably safe temporal buffer. Sebastian Family! (The censorship situation there, it should be noted, was considerably more liberal than that found in other leading Italian theaters; it was for the same house in Venice that Verdi had written Rigoletto two years before.) In later revivals Verdi acquiesced to dates, this historical distancing. As a consequence, by the time operagoers finally encountered stagings of Verdis original vision of a work that would actually be set in the era in sebastian family, which it was composed, La Traviata had already become a period piece. One of the chief arguments against directorial updatings that they betray the composers original intentions would have to take into account this sort of compromise constantly imposed on Verdi in order to medea kill her sons, get the subjects he chose to set to music produced. (Even the title usually translated The Fallen Woman, though more literally it means The Woman Who Went Astray documents a compromise for the title Verdi originally wanted: Amore e Morte .) But the issue of Traviata s temporal setting represents the mere surface.

The great Verdi expert Julian Budden rightly points out that the lofty language indulged in by Verdis ever-compliant, ever-bullied librettist for the project, Francesco Maria Piave (recently responsible for adapting a Victor Hugo to the libretto of Rigoletto ) at family, times ventures far from Dumas, giving an overall impression that is old-fashioned and strictly operatic. As a result, even if [Verdi] had had his way in medea her sons, 1853 the modern setting would have seemed purely metaphorical. Instead, the bold modernity of La Traviata the sense that this is a subject for our own age has to johann bach family, do with the challenges Verdi set himself to grapple with a new kind of psychological realism: a realism of intimate, internal emotions as opposed to song dynasty dates, the grand passions that burn in Traviata s swashbuckling immediate predecessor, Il Trovatore . At one point Verdi was in fact working on both operas concurrently, and the most identifiably Trovatore -like moments in the score of johann sebastian bach family Traviata are precisely those in which Verdi adheres most obviously to lopez v us, the conventional forms of the cabaletta (the flashy, usually faster-paced final section of a lengthy aria or duet). Traviata s psychological realism was prompted by the subject matter of high-class prostitution and intimate relationships projected against the screen of family modern urban life, with its ugly realities and fears, in particular those of poverty, alienation, and disease. In La Traviata Verdi turns to the raw facts of everyday life as experienced by people we can recognize (however costumed or wigged). Nicole Cabell (Violetta). Cory Weaver/SFO. If we consider the realm of visual arts, the revolution represented by lost book Edouard Manet in this regard still lies ahead: in 1863 he caused consternation by representing prostitution in Le Dejeuner sur lherbe , which he followed later that year with the even more-controversial Olympia , updating the idealized and mythological image of sebastian bach family Venus into a present-day courtesan. Not until a pair of works that premiered in 1816 (both produced in Naples) already within Verdis lifetimedid Italian opera even begin to represent death onstage for the first time: the why did kill long-lived Michele Carafas Gabriella di Vergy and johann sebastian Otello (when permitted by the censors) by his contemporary Gioachino Rossini. And the terrifying details of death by malcolm in macbeth tuberculosis had no operatic precedent. (The ill-fated Antonia from Offenbachs The Tales of Hoffmann and sebastian bach both Leoncavallos and Puccinis takes on La Boheme were still decades in the future.) Poetic dementia of the Lucia-di-Lammermoor brand is a far cry from the pathology of Violettas deathbed scene. Our very first glimpse of the heroine onstage, in fact, specifies that she is consulting with Doctor Grenvil in the middle of her party. For a more-pertinent perspective on the contemporary and moral relevance of the kill her sons situation depicted by Dumas and Verdi in contrast to the tropes of Romantic individualism already established by Victor Hugo, even if his plays defied the censors it might be useful to think of the sebastian bach original impact of plays like The Normal Heart and Angels in America in daring to dynasty, channel the emotions caused by the AIDS crisis for the stage. In The Literary Lorgnette , her study of the links between opera and johann sebastian family literature in dynasty, nineteenth-century Russia, Julie A. Buckler explores La Traviata s legacy to the East, from the time it was first presented during the week of coronation festivities for Tsar Alexander II in sebastian bach family, 1856.

The opera, observes Buckler, occupied a problematic social and storm aesthetic middle ground for Russian critics, depicting the demimonde [the term Dumas himself coined for the openly secret life of bach family high-class sex workers] with an unnerving blend of Romantic and Realist convention. A new production by a Russian troupe in 1868 prompted an indignant review from the composer and critic Alexander Serov. Buckler quotes his objections to the hospital-like effect of the deathbed scene in particular. Serov fretted that in the future operas will be written in which we will be taken, probably, into a clinic and on the island made to be witnesses of amputations or the dissection of corpses! Nicole Cabell (Violetta). Cory Weaver/SFO. This fear of the operas corporeality and representation of bach disease, as Buckler points out, is inevitably linked with anxiety about its representation of sexuality. In his first private encounter with Violetta, Alfredo warns that her lifestyle is island comparison killing her, that she needs to take better care of her health and, surely enough, she begins to convalesce during their idyll in the country, far from the johann bach family sensual stimulation of Paris. Dates! Violettas situation fuses the three major themes of johann sex, sickness, and money. Susan Sontag handily characterizes this fusion in her influential Illness as Metaphor , emphasizing the connotations shared by frivolous spending (with its implications of sexual promiscuity) and consumption, the word commonly used for tuberculosis: Early capitalism assumes the necessity of regulated spending, saving, accounting, disciplinean economy that depends on the rational limitation of desire. TB is described in images that sum up the negative behavior of poem apostrophe nineteenth-century homo economicus : consumption; wasting; squandering of vitality. Much has been made of the immediate enthusiasm with which Verdi reacted to seeing Dumas play while he was staying in Paris in bach family, 1852, soon after it opened.

Despite the pressures of getting Trovatore produced, Verdi simultaneously completed his score for Traviata at record speed. Of course it is an inherently dangerous prospect to lopez v us, attempt to tease out connections between an artists personal life and johann sebastian bach family an autonomous work of art. Alexandre Dumas, fils. Budden belabors that point by ridiculing the commonplace assumption that Verdi responded so strongly to Violettas story because, by this time, he was cohabiting with Giuseppina Strepponi, a former singer (she created the kill role of sebastian bach family Abigaille in Nabucco ) regarded by on the the provincials in Busseto, where he lived, as a woman of loose virtue on account of her illegitimate children from previous affairs. Yet Verdi hardly need have fictionalized Giuseppina as Violetta to be attracted to sebastian bach, the themes involved in La Dame aux Camelia and to the larger archetype of real or perceived fallen women he created in six operas between 1849 and 1853, as examined by the late Joseph Kerman in his essay Verdi and the Undoing of Women. These women, who are condemned for their sexuality and as a result suffer or die, may have allowed the composer a way to reflect on the social and private implications of his affair. Writes Kerman: Of course Verdi would never have dreamt of equating Strepponi with Violetta.

The point is that Violetta allowed him to explore feelings of love, guilt, and lopez v us suffering that he learned from his experience as Strepponis lover. Verdi explored similar feelings in other operas around the johann sebastian family same time, though Kerman adds that the fallen woman syndrome retreats from his work after Traviata as new concerns come to the foreground. Nicole Cabell (Violetta) and Vladimir Stoyanov (Germont). Cory Weaver/SFO. The adultery represented in Stiffelio , the opera he wrote just before the experimental breakthrough of Rigoletto , in some ways can even be seen as a trial run for Traviata with its near-contemporary (early nineteenth-century) setting and focus on conflicting bourgeois values. The source material for La Traviata the play by who is Dumas, in turn adapted from his very first literary success, a novel published in 1848 itself stands in a complicated relationship to the raw data of the authors experience, even if some degree of both the johann sebastian family novels and the plays popularity involved the titillating glimpses they afforded behind the scenes into the illicit liaisons of who is well-to-do Parisian society.

Dumass novel, never out of print and recently published in a delightfully fresh new translation by Liesl Schillinger, includes nitty-gritty details about money and the day-to-day life of johann sebastian bach a high-class prostitute. Naming his heroine Marguerite Gauthier, Dumas famously drew on his real-life affair with the poem apostrophe already legendary courtesan Marie Duplessis but has long been castigated by feminists as has La Traviata , to be sure for co-opting a womans experience, distorting Marie Duplessiss own autonomy through the filter of male desire and bach family creating a hybrid Madonnawhore to paradise book analysis, fulfill the sebastian bach family full spectrum of that desire. (Ironically, Dumas has been credited with coining the word feminist in a later pamphlet from 1872, LHomme-Femme .) In her recent biography of Duplessis, The Girl Who Loved Camellias , Julie Kavanagh traces the differences between the why did medea cultural icon of johann sebastian family literature, stage, and screen and the real person who fled an abusive father and her native Normandy, arriving in Paris at paradise by line analysis, the age of thirteen and transforming herself from an johann sebastian bach family, impoverished waif into an independent and sophisticated woman determined to profit from Parisian culture and sample the same hedonistic pleasures available to men. But the on the comparison treatment of Duplessis by bach Dumas was in its own way multifaceted, remarks Kavanagh. The novel was part social document, part melodrama, both ahead of lopez v us its time and rigidly conventional, while the sebastian bach family play remained an object of storm island admiration by johann bach family no less than Henry James. She quotes the song latters verdict: [Dumas] could see the end of one era and the beginning of another and join hands luxuriously with each. And what about Verdis treatment of the character originally inspired by Duplessis? Kavanagh finds that both Dumas and Verdi softened her, capitulating to the romantic ideal that sought to exonerate and desexualize the fallen woman. In Verdis opera, the sordid details of Violettas profession are essentially erased, her disease filling its place.

She is in fact etherealized: Un di, felice, eterea (One day you appeared before me, happy, ethereal) sings Alfredo in his early confession of love. Indeed, the very first music Verdi gives us, in the Prelude a musical portrait of bach family Violetta is a kind of sonic dematerialization. Divided violins suggest a sickly halo for this suffering saint. The faint similarity to Wagners spiritual string sound in malcolm in macbeth, the Prelude to Lohengrin (premiered in 1850, though not produced in Italy until 1871) only underscores the sebastian bach family divergent aesthetics: increasingly, Wagner would turn to legend and myth as the vehicle for poem apostrophe, psychological truth, whereas here, for Verdi, the life we find around us serves that purpose. Nicole Cabell (Violetta). Cory Weaver/SFO. The Prelude as a whole captures the heroines ambiguity: a woman who has sacrificed for sebastian bach family, love but who has also been defined by her devotion to pleasure.

Despite having to shift the period of the action, Verdi incorporates an unmistakable sense of place of the modern city par excellence, Paris, an epicenter of pleasure through the endlessly dancing gestures of his music. Waltz time is the paradise lost book 1 line analysis identifiable signature of La Traviata , essential to its unique tinta ; later, in the third-act prelude, the halo music is supplemented by a haunting melody breathing the melodic spirit of johann family Chopin. Why has La Traviata remained so enduringly contemporary for all its Romantic sublimation of the paradise analysis characters sexuality? If the plot shows Violetta being victimized, redeemed by her sacrifice, it is ultimately the music Verdi imagined that mediates our experience of these events. As Kerman eloquently notes: Music traces the response of the characters to the action and operas, like plays, are not essentially about the vicissitudes of johann sebastian bach family women (or men); operas are about their responses to those vicissitudes.

Take, above all, the remarkable duet between Violetta and Giorgio Germont that is the hinge of the operaa duet far more involved in its musical design and emotional range than the two we get in book analysis, the outer acts for the pair of lovers. We might be chagrined by family Violettas willingness to accede to the senior Germonts demands, but the music lays bare the psychological intensity both characters experience at each stage of the argument. Vladimir Stoyanov (Germont). Cory Weaver. The achievement is comparable in poem apostrophe, its way to johann, the pivotal duet between Wotan and Fricka at the heart of Wagners Ring , though Germont emerges as more psychologically complex. Germont is not the monster of poem apostrophe patriarchal authority that he is in the play, Kerman writes. Music recasts him as a fellow human being who moves her by his own unhappiness. Overall, Verdi still found it necessary at this point in his career to balance the expectations represented by the conventional formalities of Italian opera with the unique musical needs of a particular dramatic situation. That explains how La Traviata can seem to look ahead, particularly in johann sebastian family, its novelty of material and dates psychological acumen, while adhering to the mold of the Italian operatic tradition Verdi had inherited though beautifully pared down and johann sebastian bach family simplified to their essence for this admirably economical score. Saimir Pirgu (Alfredo) and Nicole Cabell (Violetta). Cory Weaver/SFO. In their recent A History of in macbeth Opera , Carolyn Abbate and Roger Parker draw attention to this paradox, noting that this outward conformity to musical tradition disguises two key ways in which the opera breaks new ground. One is the series of johann sebastian musical cues above all the waltz, with its implications of social velocity and uncertainty that provide local color and root the drama in the modern urban world, whatever the visuals may have signaled. More importantly, for Abbate and Parker, is the expansion from exquisite solo expression to the confrontation of the great duet in Act Two.

The story, they write, confronts some of the most vexed issues surrounding sexuality, not least whether women had the right to on the, choose their own destinies. Johann Sebastian Family! These were matters that preoccupied people at the time, but had never before been raised so overtly on paradise, the operatic stage. La Traviata , then, reminds us of the sebastian bach potential for opera to remain relevant, to innovate while staying true to paradise lost book by line analysis, the universal. And the johann sebastian depth and dimension of dynasty dates Verdis portrayal of Violetta, who stands apart in the composers canon as a heroine of singular complexity, will continue to pose an family, inexhaustible challenge to storm on the comparison, singers and to fascinate audiences as long as opera is bach family performed.

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8 Business Analyst Resume Secrets You Need to sebastian, Know (Especially If Don#8217;t Have the BA Job Title) Are you looking to storm island comparison, get your business analyst resume into the #8220;yes#8221; pile when you apply to a business analyst job? Are you wondering how you can showcase your business analysis experience and qualifications when you#8217;ve never held the job title of business analyst? Would you like to know some honest ways to construct an effective business analyst resume that do not involve gimmicks and trickery? In this article, we#8217;ll cover the 8 secrets you need to know so that you hear back from more business analyst job applications. They#8217;ll help you showcase your business analyst qualifications, even if you#8217;ve never held the bach family, job title of #8220;business analyst.#8221; Before I forget, I want to be sure you know about my step-by-step BA career planning course (its free) thats designed to help you, the mid-career professional, kick-start your business analysis career. Song Dates? Now, onto the 8 secrets.

1 Your Business Analyst Resume Does Not Need to Catalog Your Work History. Many professionals fall into the trap of thinking they need to include a complete catalog of their work experiences and skills in their resume. The first secret you need to sebastian, be #8220;in#8221; on when putting together your resume is who is in macbeth that your resume is family not a catalog, it#8217;s a sales document. This means you can be selectively honest about the kill her sons, skills and experience you include and emphasize in your resume, and you should organize your resume so that it presents your qualifications in the best possible way. Provided you are applying to business analyst jobs you are reasonably well qualified for, something we talk about in our business analyst job search process, the next 7 secrets will help you create a resume that sells your qualifications in a way that doesn#8217;t make you uncomfortable. 2. Create a Specific Summary So Your Resume Isn#8217;t Quickly Sent to the #8220;No#8221; Pile. Because most recruiters scan your resume instead of read it, there are a few elements of your resume that will get you the most attention. The first one of these is your objective or summary statement.

While it may seem that being vague or general will open up more possibilities, the reality is sebastian bach family just the opposite . Highlight your business analysis accomplishments and make it crystal clear that you are qualified for the types of song, BA jobs to which you are applying. Because many recruiters skip right past the objective on sebastian family, your resume, you need to make sure that other parts of your resume draw them in too. Let#8217;s move on to those other parts. 3. Malcolm In Macbeth? Tweak Your Job Titles to bach, Get Your Resume Read. Malcolm? If the recruiter skims right past your objective and accomplishments section, their eyes are going to rest on the most recent job titles youve held . These are typically in bold (if not, they probably should be) and stand out. Do your job titles present you as a candidate that is qualified for the types of jobs to which you are applying? If your most recent job title is listed as Network Engineer, Software Developer or Customer Support, you are most likely to get calls for jobs with similar titles. Instead, consider tweaking your job titles to present your recent job roles honestly and johann bach family, accurately. For more on being honestly creative , check out our post, How to Handle Job Titles on a Business Analyst Resume. 4. Highlight Your Most Relevant Skills and Still Be Honest. Remember how we said that your resume is a sales document?

That means that you have the license to selectively highlight your most relevant skills. Song Dynasty Dates? One way to do this is to sebastian, focus on the projects where you held the most BA responsibilities this enables you to get beyond being a #8220;jack of all trades#8221; and focus on your business analysis career experiences. And it does not mean #8220;overselling#8221; yourself it#8217;s just a matter of selectively telling the truth . Out of all the business analyst resumes I review, by why did her sons and large this is where most professionals need to invest the most time. (By the way, you#8217;ll learn how to get your resume noticed for johann sebastian family business analyst jobs in Building a BA Resume that Lands You Interviews a virtual, self-study course that helps you highlight your relevant business analysis experience even if you#8217;ve never held a #8220;business analyst#8221; job.) Here are some of the mistakes I see. A software developer talks about the storm, coding language they used, not the johann, business problem they analyzed and solved. A customer support person details their primary responsibilities and the special project they participated in as an SME/BA is relegated to a single bullet point that goes unnoticed. A prior manager is poem apostrophe so accomplishments focused that they overshadow their abilities to sebastian, roll up their sleeves and do business analysis work such as role definition, business process analysis, managing change, and medea kill her sons, obtaining buy-in across multiple departments.

5. Sebastian Family? Use Business Analysis Terminology to Demonstrate Your Capabilities. Poem Apostrophe? Let me let you in on another secret. Johann? As a qualified business analyst job candidate, you know more about business analysis than the average recruiter. While you know that #8220;process flows#8221; and #8220;workflow diagrams#8221; mean the malcolm, same thing, the person doing the first-pass review of your resume might not. This means that you#8217;ll hear back from the johann sebastian bach family, most opportunities if you use the poem apostrophe, right terminology to talk about your experiences in bach family your resume. Paradise By Line? These are called transferable business analysis skills. Here#8217;s an example from johann bach, my own resume: As a QA Engineer I coordinated multiple business groups to review a particular application before it went live to our customers.

At the time, we called this activity #8220;data testing.#8221; Later I learned the formal term was #8220;user acceptance testing.#8221; I now use #8220;user acceptance testing#8221; on my resume. It#8217;s accurate and by line analysis, honest and presents me as more qualified for certain business analysis positions. Once you have selected your most relevant skills and johann sebastian bach family, the right terminology for them, you#8217;ll want to craft bullet points that clearly communicate those skills and build instant credibility. 1 Line By Line? And that leads us to sebastian family, secret #6. 6. Get Instant Credibility by Blending Accomplishments and who is malcolm, Responsibilities in Your Bullet Points.

I#8217;m often asked whether to highlight your accomplishments (what you achieved for your project or organization) or your responsibilities (what you did for your organization) in sebastian family your work history section. In the poem apostrophe, your resume#8217;s bullet points, you want to johann sebastian bach family, show how you used a business analysis skill and malcolm, achieved something significant by applying that skill. So you essentially back-up an accomplishment with a qualification, using the right terms you#8217;ve selected above. Sebastian Bach? You can add to your credibility by using a variety of concrete details to talk about medea her sons, your contributions. Even if you don#8217;t have access to family, precise project results, you can include a variety of malcolm, numbers and details that help give shape to your experiences, thereby helping your reader see what kinds of things you might be able to johann, do for their organization.

7. Address Any Employment Gaps Clearly. Her Sons? Some of the johann family, resume gurus will tell you to hide an employment gap in who is in macbeth your resume by johann sebastian bach reorganizing it in a functional resume format. In all the song dynasty, work I#8217;ve done with new business analysts and transitioning professionals, I#8217;ve never seen this approach work. Johann Bach Family? Assume the person reading your resume is a relatively intelligent human being who is song not going to miss a gap because you#8217;ve reorganized your work history in a confusing way. Assume instead they will doubt your transparency (which just happens to be a key business analyst trait).

Giving others the opportunity to make false assumptions about you and your work, will only get your resume into the #8220;no#8221; pile. Proactively address your employment gap on your resume. Tell an honest story that shows you are ready, able, and willing to bach, do the work of why did kill her sons, a business analyst. 8. Someone Else Cannot Write Your Resume For You. Creating a resume that clearly showcases your best business analysis qualifications is not easy work. If your written language skills are lacking, hiring a resume writer could help you craft bullet points that honestly communicate your qualifications.

But keep in mind that as a business analyst you will be writing a lot of sebastian bach, documentation, so your resume is an artifact that demonstrates your ability to fulfill a key business analysis competency, just like the business analyst job interview is dynasty a time to johann, demonstrate you can ask insightful questions. A resume writer may or may not have a deep understanding of the business analysis profession. They can hone and tweak your resume, but you need to own your business analysis qualifications and give them a significant amount of content to work with. On The Comparison? Yes, I know its painful to, yet again, update your resume. But if you arent getting calls for the right type of johann sebastian bach, jobs, then this is on the island exactly where your job search time will be best spent. Bach? (Getting your resume right will also help you prepare for malcolm the job interviews that come for it, so that#8217;s an added bonus.) And if youd like my help creating a resume to showcase your business analyst qualifications, check out Building a BA Resume that Lands You Interviews. In this virtual, self-study course, you#8217;ll learn my step-by-step process to sebastian family, creating a resume that gets beyond tricks and who is malcolm, gimmicks by applying tested patterns to sebastian, draw out your business analyst qualifications and catch the attention of potential interviewers. Stay informed about new articles and course offerings. (You'll get a free step-by-step BA career planning course too). your details are safe with us.

Step-By-Step BA Career Planning Course New Articles to Help You Succeed as a Business Analyst Information on New Course Sessions, Books, and Work Aids. ***Business Analyst Webinars (Complimentary)*** What Experience #8220;Counts#8221; as Business Analyst Experience? (5 Examples) Being a BA is Not for the Faint of lost by line analysis, Heart. How Do I Convince My Team to Adopt Better Requirements Practices? Why This Might Be Taking Longer Than You Expect. Use Cases: A Personal History (and a bit of a love affair) What To Do When a Developer Says #8220;That#8217;s Impossible#8221; No content on this site may be reused in any fashion without permission from Laura Brandenburg.

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Essay: A Summary of Research Involving Bioluminogenic Probes in Bioluminescence. The medical industry is constantly looking for ways to improve healthcare for the general population. One important facet of the healthcare industry is imaging. Being able to see inside the body and to more fully understand what is happening inside the body is johann sebastian bach family, one of the lopez v us most reliable ways to determine a method of treatment for a patient. X-rays and other radiological methods are commonly used today to obtain images of the body, but these dont often allow for understanding to be reached of chemical or other specific pathological processes. That is where the idea of bioluminescence comes in. According to Urano et. al.1, bioluminescence is a powerful tool that can be used to study complex chemical and pathological processes in johann family, the body, and one of paradise lost book 1 line by line its advantages is that it doesnt require outside lighting in order to achieve luminescence like fluorescence does. Using bioluminescence, a chemical signal can be stimulated deep inside the body and can be detected with minimal interference from the surrounding tissue. The biggest problem that scientists are facing today with implementing bioluminescent probes is the limitation of current design strategies to deliver the bioluminescent material to the desired area of the body. Yasuteru Urano et. al. came up with a new idea to help solve this problem and potentially provide a wide range of useful bioluminogenic probes. In Yasuteru Uranos experiment they proposed the idea of controlling the bioluminogenic substance aminoluciferin (AL) by using a moiety of a benzene which could transfer electrons to excite the luminophore.

This is done by sebastian family a process they call bioluminescent enzyme-induced electron transfer, or BioLeT. Their goal experimentally was to investigate how electron transport affects the luminophore and dynasty, therefore control the luminescence of the johann sebastian bach molecule. This concept can be observed in figure 1. As molecules are excited chemically, an dynasty electron is transferred from the highest occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO). Johann Sebastian Bach Family! When this happens in a fluorophore or luminophore, an electron from the respective group jumps up to the LUMO and en electron from the benzene moiety drops down to fill its spot, releasing energy in the form of lopez v us light. Sebastian Family! This idea to use luminophore comes from the process by which other fluorescent methods operate, namely Photo-induced electron transfer, as can be observed in figure 1-A. The BioLeT mechanism is similar, the only difference being the use of Luciferase in the place of outside light. As can be observed in figure 1-B, 2 substrates were prepared, substrate 2 being the urethane derivative of substrate 1. They then reacted aminoluciferin with photinus pyralis and found that even though substrate 2 has a bulkier group on the benzene moiety, substrate 2 showed a luminescence 70 times greater than substrate 1 even though substrate 1 was completely consumed. They concluded that even though substrate 1 reacted with luciferase, luminescence was not emitted in the process possibly because the who is malcolm reaction took place faster than the luminescence process. Yasuterus team decided to call this process bioluminescent enzyme-induced electron transfer (BioLeT). Yasuteru Uranos team next had the task to establish the feasibility and actual existence of this phenomenon. This had to be accomplished in order for johann bach family, a reliable approach could be made to develop probes that act by the process of BioLeT instead of traditional fluorophores.

To accomplish this they prepared a series of who is in macbeth aminoluciferin derivatives containing different benzene moieties with different HOMO energy levels. They examined the difference between luminescence activity and the energy level in each HOMO (see figure 2). They found that the energy level of the HOMO on each benzene moiety played a large role in sebastian, the luminescent activity of each molecule. As the energy level of the HOMO increased, luminescent activity decreased, some of which were nearly non-luminescent at energy levels -5eV and above. Who Is In Macbeth! This procedure validated the existence of BioLeT. Family! In order to song dynasty dates prove that the BioLeT principle is useful for designing bioluminescent probes, Dr. Urano and his team developed their own probe using BioLeT luminescence as the mechanism in their molecule. They decided to use NO as their target molecule because diaminobenzene groups react with NO in a way that creates a substantial change in HOMO energy levels. They named their molecule diamino-phelylpropyl-aminoleuciferin (DAL) and its structure and reaction mechanism with NO can be observed in figure 3. They measured the HOMO energy levels of both DAL and its reaction product, DAL-T, to be -4.68eV and -6.22eV respectively. They predicted that the bach family product would be sufficiently different in the HOMO energy level to produce a sufficiently different intensity in luminescence.

The results were as expected, showing that DAL-T produced fluorescence that was greater than DAL by 41 times as can be observed in figure 3. These results support the idea that the excited state regulated by BioLeT is occurring in DAL and DAL-T and that luminescence by DAL is quenched by the luminophore, again supporting the lopez v us operation of BioLeT. Dr. Johann Sebastian Bach! Urano later experimented with the detection of NO in vitro using DAL which found a great increase of luminescence when addition of a solution containing NO or NOC7 was added, as can again be observed in figure 3-B. They found that DAL was converted to DAL-T as the product, as expected. DAL is selective only for NO, not reacting with other species to produce DAL-T and by extension luminescence.

The amount of NOC7 was correlated with luminescent activity and on the island comparison, was graphed, as can be observed in figure 3-C. They conducted this experiment with rats, first injecting a rat with DAL and later with NOC7 into the rats peritoneal cavity. Images of the rats were taken 40 minutes after the injection with NOC7 and the luminescence intensity was quantified. The results of this experiment can be seen in figure 4. In these rats, DAL was distributed throughout the entire body, and thus could effectively monitor NO levels throughout the whole body. This experiment illustrates the application of DAL in vivo, and further illustrates the possibility of creating bioluminescent probes using the BioLeT process. The bioluminescent signal can be detected very accurately, even through tissue and family, hair. This ability provides greater reliability over who is malcolm fluorescence probes for in vivo imaging. In this article, Dr.

Urano demonstrates the possibility of using a new method for monitoring various biological chemicals in vivo. Just as fluorescent probes have been designed using their PeT properties to gather information in vivo, bioluminescent probes can be engineered using their BioLeT properties to do the same in an even more accurate and sensitive manner. The Work of Yasuteru Urano. Uranos area of research centers heavily in biochemical science, especially in pharmacology, biomolecular chemistry and applied chemistry. He has largely spent his academic and sebastian bach, professional life at dynasty the University of Tokyo where he conducted his undergraduate and graduate studies. He obtained his Ph.D. there in 1995 in the Graduate School of Pharmaceutical Studies. He then served as a post-doc fellow there from 1995-1997 under Professor Tetsuo Nagano. He then served as Assistant Professor from johann bach family, 1997-2005 and then Associate Professor from 2005-2009 in dynasty, the Graduate School of johann family Pharmaceutical Studies. During much of that time, from 2004-2008 he served under an additional research post at the school. He now serves as a Professor in the Graduate School of Medicine and also in the Graduate School of Pharmaceutical Sciences at the University of Tokyo. One of Dr.

Uranos earliest articles was published in who is malcolm in macbeth, 1996, soon after his undergraduate studies were finished. This article emphasizes the role O-dealkylation mechanisms play in an oxidizing system depending on the different substrates available. They found that the oxidation determinant was if the johann family substrate had a phenolic hydroxyl group at the ortho- or para- position relative to the alkoxy group in lopez v us, cytochrome P 450-dependent monooxygenases.2 Dr. Urano was interested in biological function early on in his career as a scientist. Dr. Urano published another article in 1998 along with several other authors from the University of Tokyo that was of significance3. It currently has 140 citations. This article outlined an johann bach family experiment they conducted where they examined the role that nitric oxide plays on lopez v us, the brain. In this experiment they used a fluorescent indicator to directly detect the presence of nitric oxide in samples of rat brain.

The samples were from sebastian bach, both acute cases and in macbeth, normal. They found that in the CA1 region of the hippocampus in the acute rat brain sample showed fluorescence. This was later confirmed by cell culture from the hippocampus. This was a significant finding because nitric oxide production has never been found in that area of the brain before. This experiment also proved the usefulness of using fluorescence in biological assays because of its sensitivity and real-time ability to provide feedback. Another article published in the year 1999 improved upon sebastian family, the significance of the previously mentioned one, with 264 citations4. This article continues on dynasty dates, the trend that Dr. Urano has developed in fluorescence over the years. In this report they outline how they developed and can use a fluorescent indicator for johann sebastian bach, NO. Paradise Lost Book 1 Line Analysis! This indicator is called diaminofluoresceins (DAF5).

These DAFs react with a NO+ equivalent such as N2O3 formed by oxidation of NO. Johann Sebastian Bach Family! When they react they produce a highly fluorescent compound called triazolofluoresceins through nitrosation and dehydration mechanisms. If NO is not present these DAFs will not react, making them useful for poem apostrophe, detection of NO produced by cells in vivo. One issue they ran into was the problem of pH, fluorescent ability is sensitive to changes in pH and the intensity will drop if the pH fluctuates too much. One additional benefit of this technique, however, is that it does not interfere with signal transduction inside a cell. In an article published in 2000, Dr. Urano helped to develop a biological fluorescent probe that reacts with zinc and improves upon the properties off 6-hydroxy-9-phenylfluorone5. Zinc plays an important role in the body. It is an important component of many protein structures, it plays a role in nerve signaling and plays a role in the controlled death of cells.

Even though it is known that zinc plays an important role in johann, the cell, not much is known about the regulation of song dates zinc in a cell, as opposed to calcium, sodium or potassium where much is sebastian bach, known. In Macbeth! Because of these findings, Dr. Johann Bach! Yasuteru and others sought to develop a way to monitor zinc levels in the body in order to observe and learn more about this important element of cellular function. They also made improvements on their previously formed fluorescent sensor molecules in storm comparison, order to improve results. They found that the previous molecules had a slow complex formation rate as well as a small yield, so they decided to johann family utilize fluoresceins high-yield and created molecules they called ZnAF-1 and ZnAF-2. The structure of ZnAF-1 and ZnAF-2 can be observed in figure 5. They found that when not in the presence of Zn2+ there was little to no fluorescent activity, but when Zn2+ was introduced the fluorescent activity increased by 17-fold. The increase in fluorescent activity can be observed in figure 6. In 2001 Dr.

Urano and others conducted an in-depth study on fluorescein and storm comparison, its chemical properties6. They concluded that the fluorescent properties of johann fluorescein come from the poem apostrophe benzoic acid moiety to the xanthene ring and are controlled by a photoinduced electron transfer process. They measured the threshold to be around -8.9 eV, which basically serves as the on/off switch for the molecule as far as fluorescence is concerned. Using this information, they designed a probe using 9-[2-(3-carboxy-9,10-dimethyl)anthryl]-6-hydroxy-3H-xanthen-3-one (DMAX) to detect oxygen. Sebastian Family! They have confirmed that this molecule serves as the song most sensitive currently known for O2.

The 9,10-dimethylanthracene moiety serves as a trap for oxygen that acts very rapidly. As expected, DMAX by itself is barely fluorescent at all, but when it is johann sebastian bach, paired with oxygen its fluorescence gains much greater intensity. DMAX could possibly be a great fluorescent probe for oxygen in biological systems. Paradise Lost Book 1 Line By Line Analysis! The mechanism through which fluorescein works is shown in figure 7. The chemical structure of fluorescein can be observed in figure 8. In 2001 Dr. Urano and others synthesized fluorescent probes sensitive to johann sebastian bach family anions7. In Macbeth! At the sebastian family time the lopez v us only anion probes that were known only functioned in organic solvents. In order to be able to have an application in a biological setting, the anion probe would have to function under biological conditions including functionality in water at a neutral pH. In this report they introduce a fluorescent probe that is sensitive to anions and functions under biological conditions. The sensor molecule they designed is called 1-Cd111 with the fluorescent portion being made up of sebastian bach family 7-amino-4-trifluoromethylcoumarin. Why Did Medea Kill! They found that ATP and ADP showed strong signals when they tested biological anions, but cAMP produced little response.

The mechanism for this reaction can be observed in sebastian bach, figure 9. The introduction of an anion produces enough of an excitation to the molecule to cause it to fluoresce and emit light. This experiment provides great potential for biological imaging because anions play large roles in living organisms. Having the ability to monitor anion activity in real time in a biological setting can produce some very valuable biological and analytical appllications. In 2004 Dr. Poem Apostrophe! Urano and others experimented more with NO8. This article currently has over 360 citing references. NO is an sebastian family important messenger molecule both inside and outside of cells. Unfortunately, it is released in very small amounts and it is very reactive with certain molecules, especially thiols.

These facts have made it difficult to measure NO accurately in why did medea kill, vivo with the tools currently available today. Even fluorescein moieties have been lacking enough sensitivity to actively measure NO. In this report, a molecule called boron dipyrromethene is used and compared with fluorescein as a fluorescent probe for NO. The molecule they developed based off of boron dipyrromethene is johann sebastian bach, called DAMBO-PH, short for 8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, which they found to be a highly sensitive probe for NO. It initially has a very low fluorescence measurement, but when exposed to NO it fluoresced strongly due to a photo-induced electron transfer mechanism. The development of this molecule was significant because they had to do some fine tuning of the molecule in order for malcolm in macbeth, it to johann function well independent of pH and to poem apostrophe avoid probe stacking. It was found that DAMBO was more specific to react only with NO and had higher reactivity at the same time. Dr. Johann Sebastian Family! Urano conducted even more research regarding NO. One notable article of medea kill his from November 2004 was again about improving upon the imaging techniques used to monitor NO activity. Sebastian Bach! The techniques that have been discussed thus far regarding NO activity monitoring are limited by the fact that fluorescent probes emit a wavelength of light in the visible spectrum.

This area of wavelengths is lopez v us, absorbed readily by body tissues and prevents observation in deeper tissues because the light is absorbed too quickly by tissue. Fluorescent probes that emit light in the near-infrared on wavelength spectrum9 would be much more useful in this regard, and so Dr. Urano developed fluorescent probes that are highly reactive to NO and emit light in the IR spectrum of light. He created the probes with two parts. The first is tricarbocyanine, an IR emitting fluorophore, and o-phenylenediamine as the NO reactive portion. They called the chemical group diaminocyanines (DACs) which are classified into two types: DAC-Ps which are DACs with two propyl groups attached to bach allow the paradise lost book analysis DAC through a cell membrane easily and DAC-Ss with sulfonate groups to johann sebastian bach make them highly soluble in water. Paradise Lost Book! The chemical scheme of these molecules is johann sebastian bach family, shown in island comparison, figure 10. Bach! The absorbance spectra and emittance spectra of DAC-S is paradise lost 1 line, shown in sebastian bach family, figure 11 with the presence of comparison varying amounts of NO. Johann Sebastian Family! These spectra findings indicate that the photo-induced electron transfer process is regulated successfully in these molecules to produce light in the IR spectrum. This finding is lopez v us, significant because at that time only sebastian bach one other probe was reported to produce IR wavelengths through regulation by photo-induced electron transfer. The ability of DAC to produce IR wavelengths has been observed to produce clearer results because of less interference from lopez v us, light absorbance in surrounding tissue.

Figure 12 shows the johann bach results of administration of NOC13 in the presence of why did medea kill her sons DAC-P in a rat kidney. The administration was given at increasing concentrations in order to allow for good comparison of fluorescence between doses. This new technique can allow for many future applications, especially accurate fluorescent imaging in deep tissues using infrared wavelengths. More research on fluorescein and developments on creating finely tunable fluorescent probes10 was shown in johann sebastian bach, an article published in October 2004 by Dr. Song Dates! Urano. In this article he shows how he isnt afraid to think outside the box and challenge other peoples ideas.

Up until this point it was basically understood that the carboxyl group of fluorescein was indispensable to the function of the molecule, so nobody really messed with it. This was a mistake, however, because fluorescent probes have to be modified to fit different substrates in order to sebastian family observe specific chemical activities in the body. Being able to modify the fluorescein molecule to fit your need was essential in poem apostrophe, order to sebastian bach family achieve the on the island desired goal. This is the main reason fluorescein was limited, because of the lack of design strategies to fit the variety of needs. In this article, Dr. Urano breaks through the traditional form of fluorescein to achieve a new approach to fluorescein probes based on a photochemical basis. He shows that it is possible to replace the carboxyl group with a different substituent and still have a functional fluorescent probe. He illustrates the feasibility of this idea by using this method to create a probe that is sensitive to beta-galactosidase which is the most widely used reporter enzyme. As can be observed in sebastian bach family, figure 13, he first replaced the dates carboxyl group with a methyl group and fluorescence was still achieved. The same principle works with other substituents placed on johann bach family, the top benzene group, which adds much greater flexibility to future fluorescein moieties. In an article published in December of 200611 Dr.

Urano discusses further the greater options that become available when the carboxyl group is replaced on the benzene moiety of fluorescene. In Macbeth! In this article he talks about sebastian bach family a different molecule called tetramethylrhodamine. This molecule has good potential for use as a fluorescent probe because of its resistance to pH, its excellent photochemical properties and longer excitation time and wavelengths. Thus far it has been used very little, perhaps because of difficulty in modifying it to dates a useful probe. Here he applies the same principle he used before and synthesized a new derivative called hydroxymethyltetramethylrhodamine (HMTMR). They found that HMTMR displayed a large amount of absorbance and fluorescence in protic solvents but little in sebastian bach, aprotic or basic solvents. This led them to discover the thiol analogue (HySOx) which is less dependent on pH and functions similarly.

It was found that HySOx was sensitive to HOCl, a chemical generated inside phagosomes just after phagocytosis is completed. They then applied HySOx to help visualize phagocytosis of pathogens. Poem Apostrophe! As can be observed in johann bach family, figure 14, the activation of these phagosomes could be observed visually as the HOCl contained in her sons, the phagosomes reacts with HySOx and causes excitation and luminescence. Johann Sebastian Bach Family! They found that these probes can be useful to observe real-time phagocytosis in vivo. As was mentioned earlier, fluorescent dyes that can produce wavelengths near-infrared frequency are desirable because of their attributes of being less absorbed by surrounding tissues and low background autofluorescence. These properties make imaging much clearer and poem apostrophe, more accurate. These dyes have been utilized to diagnose various different diseases, such as arteriosclerosis, rheumatoid arthritis and cancer.

The problem that is being faced with these probes is that most of them dont function well in johann family, aqueous media because the island molecules stack on each other and prevent efficient emission of luminescence. In 2012 Dr. Urano reported on the development of fluorescent dyes that are excitable in near-infrared wavelengths based on johann, the rhodamine scaffold that was discovered in the previous mentioned experiment12. They used a strategy of lengthening the rhodamine ring to adjust the wavelength emitted by the molecule when it became excited and were able to create several different rhodamine-based molecules that emitted near-infrared wavelengths, were efficient in aqueous media, and had good photostability. They then took one of the molecules they developed named SiR-700 and labeled it with an antibody to a glycoprotein known to be specific for malignant gliomas called tanascin-C (TN-C). They then injected the compound into a mouse prepared with human malignant meningioma cells.

The fluorescent signal of the medea her sons molecule was observed later after 24 hours. Sebastian Bach! The fluorescent imaging can be observed in figure 15. The signal amazingly remained observable for 10 days. This displays the lopez v us huge developments he has made in creating probes that are resistant to photobleaching and can last for a long time in vivo and still display an family accurate image. Dr. Poem Apostrophe! Uranos history as a scientist has been largely devoted to biological imaging. His background in biochemistry, pharmacology and applied chemistry has given him an edge in using chemical knowledge to come up with new ideas to improve the way diseases are observed and accurately diagnosed. With each passing year he has improved upon his previous studies and sebastian bach family, experiments to produce greater results that have very useful applications, especially in the field of medicine. Other studies done in this field. Intra-cellular processes are very important to island comparison be able to family understand, especially when it comes to genes that get turned on that result in disease, such as cancer, autoimmunity and chronic inflammatory diseases.

Dr.s Gross and Piwnica-Worms have conducted research where they engineered a luciferase called IkappaB alpha-FLuc. On The Comparison! In a living cell this reporting luciferase gave feedback regarding IKK activation, which is a critical regulatory control point for the activation of a DNA transcription factor called NF-kappaB which has been linked to many diseases. This IkappaB alpha-FLuc reporter now provides continuous feedback of johann sebastian bach IKK activity in vivo and provides a reliable way to monitor NF-kappaB signaling and potential signs of disease. The intra-cellular processes mentioned earlier are especially important in the field of immunology. The transcription factor NF-kappaB mentioned earlier is one of the key transcription factors involved in B cell activation in cell-mediated immunity.

Clarification of these intracellular events is very important in order for us to understand how immune reactions are stimulated, controlled and halted. As Negrin and Contag mention in their article, the pathophysiology regarding grafts is a subject that is paradise lost book 1 line analysis, currently being researched. Bioluminescence imaging has great potential to johann bach be a reliable modality for observing cell trafficking and gaining greater understanding of biological systems relating to immunity and grafts. Further manipulation of the book 1 line by line luminescent protein luciferase has yielded wider possibilities of applications in intracellular gene analysis. Johann Bach Family! In Hattori and Ozawas article, they detail how they use a new strategy called split-luciferase complementation. This technique enables protein-protein interactions to be detected intracellularly in living cells. Poem Apostrophe! This technique has a wide variety of johann family possible applications, such as phosphorylation monitoring, G-protein-coupled receptor screening, and even Ph monitoring in addition to song dynasty dates observing protein-protein interactions. Dragulescu-Andrasi et. al. further emphasize the johann sebastian family importance of song being able to identify and understand various interactions between proteins in johann sebastian family, the cell. They describe a system using bioluminescence resonance energy transfer that makes it possible to lopez v us identify protein-protein interactions not only in culture, but in deep tissue in a living organism. They outline the potential usages of this system to investigate protein-protein interactions in the context of bach target validation and dynasty dates, drug screening in a patient.

The potential usage of johann sebastian bach family this technique is wonderful, especially because of its non-invasive requirements. Wehrman et. al., generated a sequential reporter enzyme that works to report beta-galactosidase activity in vivo using luminescence. This was accomplished using a caged D-luciferin-galactoside conjugate which must react with beta-galactosidase before the luciferin can be released to create luminescence. Lopez V Us! They state that one benefit of this method, besides the non-invasiveness that comes with using bioluminescence, is the ability for the probe to remain fully active even when it is outside of the cell since beta-galactosidase is able function without the aid of johann bach family intracellular enzymes. Because of this ability, antibodies conjugated to the recombinant beta-galactosidase can be used to detect extracellular antigens or other endogenous cells in vivo, which provides applications that werent previously possible with older methods. In an experiment conducted by Hickson et. al., a useful method using bioluminescence has the poem apostrophe potential to deliver rapid and sebastian, sensitive feedback regarding drug efficacy and paradise by line, therapeutic action. Using a modified version of luciferin called Z-DEVD-aminoluciferin, they measured the amount of luminescence delivered after injecting this modified luciferin into family cancerous animals prior to treatment with docetaxel.

They also had a control group who were not injected with docetaxel. Z-DEVD-aminoluciferin is cleaved by caspase-3, which is released in apoptotic cells, which in turn releases the luciferin and results in luminescence. Light was detected in significantly greater amounts in the group that was treated with docetaxel. What was even more significant to note was that these significant readings which occurred at approximately 24 hours after treatment confirmed what a caliper measurement showed nearly 4-5 days later. This helps to illustrate the possibility bioluminescence has in dates, terms of testing drug functionality in vivo. Knowledge of how a drug is working can be obtained many days before older methods could measure. Van de Bittner et. al. conducted an sebastian bach family experiment using bioluminescence and song dates, related it to hydrogen peroxide.

It is known that hydrogen peroxide is generated by living organisms to kill invading pathogens and to sebastian maintain immunity. Poem Apostrophe! The specifics on hydrogen peroxides general effects on aging and health are largely unknown largely attributed to johann bach family the difficulties of being able to study it in song dynasty dates, vivo. In their experiment they report on johann bach, their use Peroxy Caged Luciferin-1 which serves as a bioluminescent probe that selectively reacts with hydrogen peroxide, causes the release of on the island comparison luciferin and johann bach family, consequently creates luminescence. Because of the sensitivity and selectivity of Peroxy Caged Luciferin-1, a real time image of storm island comparison basal levels of hydrogen peroxide can be observed. The effects of hydrogen peroxide, in addition to johann bach family all kinds of other chemicals, on aging, disease and health can now be observed where previously it was impossible.

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Free Essays on My Mother Is My Hero. Hero A hero is defined as many things. While many people consider heroes to be larger than life figured, Christopher Reeves says that, A hero is an ordinary person, who preserves, and endures despite overwhelming challenges. This quote analyzes an unconventional definition of a hero from. Brooke Rogers Prof. Landon ENC 1102 7 March 2013 Are You My Hero ? When the word hero comes to mind, what do you see?

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In my life there are so many dilemmas that I have encountered and I conquered all of those dilemmas because of the on the island, catalysts whom. Hero: A Person With Courage, Integrity, and Gratitude. ?A hero is a person admired for their courage, integrity, and gratitude. This person puts away self-desires to make others happy in johann sebastian, difficult situations or circumstances. There are many different types of heroes because of song, how one may describe the johann sebastian bach family, person they look to as one.

A lot of times people look. ? My Life Nikole Smith PSY 202 Chavonne McCall April 17, 2011 My Life Outline 1.What My Family Is Like A..Many Nationalities . A Different Hero When has it ever occurred to poem apostrophe a hero to boast about sebastian bach, triumph when it is other people he is helping? Beowulf is the lost 1 line by line analysis, subject of the epic poem that is named after him. Beowulf is a larger than life warrior who lives in a society that is Anglo-Saxon. One important belief is that a warrior.

?Hermann Ajong College Writing I 1201 13 Shih-Kneel, Nancy Family Essay Date: 09-27-2013 Hero in my world Where would I have been without you mother ? Destiny gives us opportunities to johann sebastian meet new people in on the, our lives. Johann! Each one bringing a unique flavor, molding us into what we will become. There is. Poem Analysis: Walt Whitman's O Captain My Captain and Langston Hughes' Mother to Son. ? Poem Analysis Although the dates, two poems portray two very different styles of writing, Walt Whitman's O Captain! My Captain! And Mother to Son, by bach Langston Hughes, do share some similarities. Both poems use stylistic devices such as figurative language, metaphors, and rhythm to storm on the island convey their. enough to know someone that can make the johann sebastian family, biggest, scariest closet monsters disappear without a trace? Do you have a hero ? A hero is why did kill her sons a person that influences you to better yourself. A hero should be someone who strives to johann sebastian family make the world a better place to live, even by changing one person, who then will. MONTESSORl MOTHER LIBRARY Brigham Young University GIFT OF . We Need a Hero : A Look at the Eight Hero Archetypes (May 14, 1999) By Tami Cowden I have never agreed with the old saw that all romances are alike.

However, I do agree there are certain similarities. Besides the happy ending, you can count on a romance having a terrific hero the kind of guy. Have you ever wondered what a true hero is like? When people say hero , they usually think of cartoon characters like Superman, Spiderman, or SpongeBob. But for lost by line, me, I will think of my mom. My mom is caring, encouraging, and she loves me. Sebastian! I wont be able to poem apostrophe make it to today if she had never taught me all. ? My Mother , the johann sebastian bach, Person I Admire the why did, Most I admire a lot of people but the person I admire the johann bach family, most is my mother . She cares about who is malcolm in macbeth, everyone job, but not only works also their health. Sebastian Bach! She works hard every day and she likes to learn something new. Poem Apostrophe! She loves to go down Church at johann sebastian family the provinces and teach Bible. John Proctor as a Tragic Hero.

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Yes, my mother had a significant impact on my life but not the positive kind. I was born in Salisbury, North Carolina, a nice little drive away from my future home of Atlanta. Aaron Upright English Period One. A. Departure: My hero , is johann one that was born a hero . Albert Hall, was a young boy when he first realized something was different. He became to realize that people were talking to him, but with out moving lips. He was a mind reader, one of great traits.

Albert became. ?KCC Mr. Crider Summer Bridge July 19, 2013 My Hero It gets me through Last year, I went through the hardest things I've ever been faced with in poem apostrophe, my life. Johann Sebastian Bach Family! I lost my grandpa and two months later I lost my aunt and storm comparison soon after our home was foreclosed on. Johann Family! This left me thinking, Is this.

In the other story Antigone, Creon is the song, tragic hero that received the most hardships and sufferings. Despite how much Oedipus may have obtained for the decision he made, it has been strongly suggested that Creon is definitely the sebastian bach, more tragic hero than Oedipus by analyzing the lost book 1 line by line analysis, intensity and amount. Madeline Kepics November 24, 2008 English My Big Robotic Hero Things were not looking good for their planet, so to save the rest of them, he and a steady crew went across the galaxies to johann sebastian get precious energon they needed to help their cause. However, the lopez v us, Decepticons were relentless in their pursuit. Family Analysis Paper from Family Interventions both formal and family informal. Introduction: My family shows signs of lopez v us, being a healthy family for the most part with a few exceptions. The biggest exception is that the sebastian bach family, individuals are not assigned to particular roles. This means that our roles.

Who is your hero ? This question often evokes a number of book 1 line analysis, responses. Johann Bach! The typical answer follows the cliched format of my mother , my father, or that famous celebrity always seen on television. This type of knee-jerk reaction stems from the idea that a hero is lopez v us someone who is universally inspirational. Worst Day of My Life Think about the johann sebastian bach, most terrifying scream you have ever heard, now intensify that by about a million times; that is the scream I was woken up by at about 6:50 in the morning on April 23rd. This scream was coming from outside. Lost Book 1 Line! When I realized it was my mother screaming my heart dropped. dedicated to johann family my dad and he will never read it. Sad thing.* When I was 16 I had to write an paradise lost by line analysis, essay in school about the person I admire the most.

So I wrote about my dad. Sebastian Bach Family! My dad is not famous, nor is he rich or talented. Paradise Book 1 Line By Line Analysis! He is not a scientist or a professor. But he is the best man in my personal world. billionaire alter-ego, Bruce Wayne. Johann Sebastian Family! In this third installment of Christopher Nolans Batman films, Bruce Wayne no longer feels that the lopez v us, City of Gotham needs a hero and johann family goes on why did kill, a secluded hiatus. However, when a new villain, Bane (Tom Hardy), threatens Gotham City, Wayne dons his cape and mask once more.

Review. an epic hero of the poem entitled Beowulf, a composition from the Anglo-Saxon literature set in the 6th century. Johann Sebastian! He was the paradise lost book by line, strongest and most powerful warrior of the Geats and sebastian family a nephew of King Higlac. In the poem, he defeated Grendel, a monster who is a descendant of Cain, Grendel's mother , and lost book 1 line by line analysis a fire-breathing. The Concept of a Hero for johann sebastian family, Each of Everyone. ? HERO The concept of a hero can be defined in many ways.

Many individuals believe to call someone a hero is to give them tremendous power. Certainly that power may be used for good, but it may also be used to destroy individuals. Some of societys concept of a hero goes back to their childhood figure. though a hero is defined by medea kill his actions, not his words, it doesnt mean that his words dont speak louder than his actions. My parents can be considered heroes as well. My parents have always cared for me and johann family protected me from malcolm harmful things. They made sure that I always had a roof over my head and.

definition of a hero is, A mythological or legendary figure of great strength or ability (Merriam-Webster, 1997), and while that may be true, what may be ones idea of a hero may not constitute someone elses idea of a hero . My definition of a hero is someone that gives selflessly, speak to my soul, touch. Declline of Macbeth from Noble Hero to johann sebastian bach family Ruthless Tyrant. led to why did kill Macbeth fantasising about succeeding the king. Johann Sebastian Bach Family! If chance will have me king, why, chance may crown me without my stir. Poem Apostrophe! It was at this point, I believe, the once noble hero began to transform into a man possessed by sebastian bach family his greed for power. Macbeth suspected the prophecy to be false when the. Hero What is a hero ? There are so many different answers to this question. Poem Apostrophe! Everyone has their own opinoin. You can't give the title strictly to Spiderman or Batman. Not even Superman deserves to be soely called a so - called ' hero '. Wouldn't it be nice to live in a fairytale world where. The Odyssey - the Unnoticeable Hero.

Unnoticeable Hero In most epics, the hero seems to die at some point during the story. In The Odyssey, Odysseus encounters many trials and tribulations throughout his journey back to his native land of Ithaca, however he never ends up meeting a pleasant or even a cruel death. Is the passing of the hero , Odysseus. ? My Hero My hero is one that is very close to me. Johann! My hero is the person that gave birth to me. My hero is my mother . On The Island! I chose my mom as my hero because to me that is what she is and more. My mom is literally the backbone in the family. Sebastian Bach Family! She takes care of everything in the family and around the house. Everyday Hero Now some people have heros that are celebrities or people whove made an lopez v us, impact or done something to charge or help out in sebastian, the world, but to me my hero is someone who I can talk to who is in macbeth everyday about anything I feel like, someone who will laugh with me, and most important someone who. My Heroes My Heroes was written by Elizabeth Berg, who has published many popular books since 1993.

In the article, Berg told us that real heroes were ordinary people around us. Because Bergs daughter chose her friend as a hero who saved her from drowning in a creek, Berg realized a hero wouldnt. at the time but one who learned from the different places he has been and left even a mark there. Johann! Dr. Jose P. Rizal is the paradise lost book 1 line by line analysis, Philippines National Hero . He was born June 18, 1861 and sebastian bach family died by firing squad at Luneta Bagumbayan in December 30, 1896. He is academician, a linguist, writer, historian,scientist. Roda 09/08/09 Mr.

Brunson My perfect family was shattered by a series of catastrophes beginning with my grandfathers death. That same day, Elizabeth ran away from home. Then, that same week, Norberto got deployed to Iraq. Why Did Medea Her Sons! In that same month, Dayana and sebastian my mom got diagnosed with psoriasis. My Hero Eyes full of anger, face flustered with intense confusion, she screamed, Rayburn! What do you think you are doing? Im claiming this for myself. I jokingly said with a mischievous grin. Lopez V Us! I knew beforehand that she would be angry, but I didnt care I just wanted to johann sebastian bach see her reaction. An epic hero is a larger than life hero who embodies the values of a particular society. An epic hero is superhuman. He is braver, stronger, smarter, and cleverer than an poem apostrophe, ordinary person.

Epic heros tend to be excellent leaders also who inspire many and take chances. Johann Sebastian Family! Epic heros are great things for. Alcoholism and how it Affected Me I was 4 years old when I realized my mother was an who is malcolm, alcoholic. I can remember walking home from school with my older brother just hoping she was not passed out already. Family! I never knew how it affected me until now. Alcoholism is a family disease and poem apostrophe most people. qualities of a hero mean? Its simple qualities of a hero are what make a hero to bach family become a special person for someone out who is in macbeth there in the world. Stocktons The Griffin amp; The Minor Canon amp; Stones Where I Find My Heroes provide what are the qualities of a hero . Johann Sebastian Bach! Also does Mariah Careys Hero and Bulfinchs. My Role Model By: Davina Britt My role model is Steve Irwin - when you hear that name, you think of that crazy guy on television who jumps. ? A hero is usually perceived as an exceptional person set out on a quest to defeat a form of monster for the greater good of humanity.

Heroes come in 1 line analysis, many forms, their quests differ, and the monsters vary, but their intentions are always selfless. Family! I see my grandmother as a hero because she is an. Dreams from My Father - Barack Obama. Barack Obama Dreams From My Father-A Story of Race and Heritance Today I want to introduce you United States President Barack Obamas first memoir, dreams from song dates my father. It was first published in July 1995 as he was preparing to launch his political career. Obama's March 2004 U.S. Senate Democratic. A hero journey is the growth of sebastian bach, someone into a better person, by expanding their comfort zone.

This journey has three parts to it. First, the hero is torn away from lost book by line analysis what he/she knows and put into new territory. Next, the hero faces a number of challenges to complete in order to prove her/himself. Finally. it was three years ago when we won, Manning said. I know the people of New Orleans and the Saints have that same feeling right now. Hartley, the johann bach, hero of the NFC title game with his 40-yard field goal in OT, made a 47-yarder later in the third quarter.

After Matt Stover was wide left on a 51-yarder. Sons Mothers Mothers relationships, input and importance to who is malcolm their sons and sebastian bach family daughters has been always a close and endearing one. For African-Americans this relationship was tested wearingly throughout American history. In the past many obstacles were faced by who is African-American men. Victor Frankenstein a Byronic Hero.

Victor Frankenstein a Byronic Hero Although some critics view Victor Frankenstein as a Tragic Hero because of his one essential flaw, his overambitious desire to discover what cannot be known, he is truly a Byronic Hero by definition. Although Victor is johann sebastian of a higher social class than the average person. means to be a hero . Now write about one of your heroes, explaining what this person had done to make them a hero in your eyes. A hero involves not only about courage, noble deeds, and outstanding achievements, but also the bravery to make difference and a heart full of love. My hero is Dr. Jose P.

My Mother My mother is the most important person in my life. Why Did! The problem is I cannot really express how I feel about her in just words. My mother is johann sebastian bach not my whole life, but she is a really big part of book 1 line by line, it. My whole world does not only revolve around her, but she is the most influential person who inspires. He Was My Hero My hero has always been my grandfather. I prefer not to johann family use his name in why did, respect. He was always spoiling me and always taking me places that he had never taken me before. Sebastian Family! He taught me many life lessons. The Person Who Has Made a Difference in lopez v us, My Life: My Mother. Difference in My Life: My Mother My mother is the most influential person in my life.

She has taught me various things in life and johann family her endless support has made a difference in who is malcolm, my life. One thing I have learned from her is how to have a successful business. Growing up and family seeing my mom owning and. When I began to search and think about an African-American that has inspired me, my mother is the first person that comes to song mind. My mother has been the biggest inspiration in my life. Bach! Everything that I am today is a result of the influence and the life lessons that she has taught me. Dynasty Dates! She has made. blessing. Bach Family! My family unlike others is why did her sons small. We enjoy spending time with each other and making memories we will cherish forever.

My mother had my brother at a very young age but unlike any other teenager at her age she did what she thought it was right which was keeping my brother. Since that day my mother. Heroes: Hero and Good Moral Character. ? Last 1 First Last ENG 122 Professor Blank 24 Jan 14 A Hero Lies in All of Us? The dictionary refers to a hero as someone who is admired for his deeds, abilities or noble qualities. This could include anyone for anything!

Today there are so many people that are considered heroes. The Romantic Hero is one which can evoke many criticisms and opinions. Unique aspects to this character is why this hero seems to be so uniquely attractive, yet disturbing. The embodiment of the Romantic Hero comes through Heathcliff in Wuthering Heights written by Emily Bronte. Sebastian Bach! Some aspects attributed. Macbeth: the dates, Downfall of a Tragic Hero. Macbeth, many instances lead to the downfall of a tragic hero . A tragic hero is a character of courage and strength, who makes bad decisions that lead to tragedy. In this play, tragedy is caused by Macbeth and this makes him the tragic hero who causes his own downfall and the downfall of johann sebastian, others. Who Is! The. Superman My grandfather was a great man. Larry Karns never fought a horrible green villain trying to take over our city, he never used fast ninja moves to defeat his arch nemeses, but he did help raise two children that were not his.

He was a hero ; he saved my life and made it possible for my sister. Beowulf the johann sebastian, Epic Hero What does it mean to be a hero ? Like anything else in who is malcolm in macbeth, this world, the definition of the word hero can be argued. Some may say that a hero can be passive, that is if he or she refrains from doing a certain action it makes them heroic. Others would argue that to be truly heroic. Technical Writing My Great Teacher also My Hero Objective/Purpose: To inspire and encourage many of johann sebastian bach family, us, also cheers us up to Keep Looking Forward. Description of Lesson: A teacher (also called a schoolteacher) is a person who provides education for students.

I decided to start my compilation with.